6^th International Conference on Clinical and Experimental Dermatology May 04-06, 2016 Chicago,Illinois, USA

Biography:

Farrokh Khosravi has completed his Fellowship of Rheumatology in Shiraz University of Medical Sciences. He is a part time Assistant Professor of Medicine. His field of interest is autoimmune skin diseases and he has published papers in the field of Behcet's disease and Multicentric Reticulohistiocytosis in reputed journals and served as a Reviewer for Journal of Hormozgan University of Medical Sciences and Rheumatology Research Journal.

Abstract:

In this talk recent advances in understanding of drug induced fractures will be discussed. Fractures are the serious problem of the aged population, which is responsible for significant amount of morbidity and mortality. Any attempt to prevent or decrease incidence of fractures will save lives. Drugs increase Osteoporosis and Sarcopenia which both have a role in increasing fracture incidence. Sarcopenia is characterized by progressive and generalized loss of muscle mass and strength.

Biography:

Leonard B Weinstock has completed his MD at the Rochester University School of Medicine and Gastroenterology Fellowship at Washington University. His research revolves around extra-intestinal manifestations of SIBO (including rosacea, restless legs syndrome, fibromyalgia and chromic pelvic pain syndromes), control of systemic inflammation and intestinal dysbiosis. He has published more than 80 chapeters, papers and abstracts. His academic position is Associate Professor of Clinical Medicine and Surgery at Washington School of Medicine in St. Louis and he is the President of Specialists in Gastroenterology.

Abstract:

Background: Rosacea is associated with Crohn’s disease, liver disease, chronic pancreatitis, achlorhydria, Helicobacter pylori and recently with idiopathic small intestinal bacterial overgrowth (SIBO). Two publications demonstrated that rifaximin, a non-absorbed, gut-directed antibiotic for SIBO led to improvement in facial rosacea. Ocular manifestations occur in up to 58% of rosacea patients and include dry eyes, foreign body sensation, photosensitivity, eyelid inflammation, neovascularization and corneal ulcers. Methods: Patients who had been diagnosed with ocular rosacea by four ophthalmologists were referred for SIBO testing using the lactulose breath test (LBT). All were refractory to rosacea therapy. An open-label, IRB-approved trial of rifaximin 550 mg/3x/day for 10-14 days was performed in LBT-positive subjects. Ten and twenty days after ending rifaximin, subjects were queried if their eye symptoms had marked, moderate or mild improvement or if they were unchanged. Results: Twenty four patients (21F/3M), mean age 59 with facial involvement in 4 were tested. The LBT was positive in 9/24 (38%). LBT-positive subjects had chronic gastrointestinal symptoms in 63% vs., 33% in LBT-negative subjects. Rifaximin was prescribed to 9 LBT-positive subjects. Insurance denied the prescription in one subject. One subject was lost was to follow up. Improvement in ocular rosacea symptoms was marked (4), moderate (1) and mild (2). Conclusions: Rifaximin therapy led to improvement in ocular rosacea in the setting of SIBO. Dysregulation of the innate immune system as a result of gastrointestinal inflammation could lead to an increase in systemic cytokines and microbial antigens/antibodies in the skin and eyelids leading to activation of rosacea.

Biography:

Professor Svyatenko Tetyana has completed her PhD at the age of 27 years from Bogomolets National Medical University (Kiev, Ukraine), has completed her Professor at the age 35 from Bogomolets National Medical University (Kiev, Ukraine). She is the director of Center Dermatology and Cosmetology of Professor Sviatenko. She is professor of Dermatovenerology department of Dniepropetrovsk state Medical Academy, Ukraine. She has published more than 300 papers in scientific journals and has been serving as an editorial board member of repute. Participant and speaker at more than 200 scientific congresses and symposia in Ukraine and abroad. Founded a school for atopic patients in Dnepropetrovsk region.

Abstract:

The skin is affected in 20-25% of all cases and is very important in the diagnosis, prognosis and course of sarcoidosis. In recent years the number of cases of sarcoidosis increased in Ukraine. We are observed men, born in 1967, complained about skin rash in the area of the upper third of the back slightly itching. The process progresses slowly(2 years). He was treated with the diagnosis: multiple warts? Lichen planus? – without effects. Patient also has a history of atopic dermatitis, androgenetic hair loss. The parents and close relatives are healthy. Physical condition was satisfactory. Pathological skin process is localized on the upper back. Rush is represented by single knots, up to 2 - 5 mm in diameter, multiple scattered and confluent lesions up to 3 cm in diameter, the colour red and pink, with telangiectasias, soft-elastic consistency, the surface smooth and shiny. On diascopy symptom of "dust", the phenomenon of "cape". Blood test results: normal range. Punch biopsy was provided. Histological investigation shown epidermis without changes, multiple epithelioid - cell granulomas are determined in papillary and reticular dermis with the presence of single giant polynuclear cells. Granulomas have clear boundaries, with the presence of single lymphocytes around some of them. When painting on Alcian blue PAS + cells of fungi have been identified. The basement membrane is within norm. No dermal mucin. The diagnosis: Cutaneous sarcoidosis. In our opinion, the case described introduce certain interest in the daily practice of a physician - clinician.This condition is rare and difficult to diagnose.

Biography:

Dr. Ylfete A. Shatri - Muçaj, a clinical dermatovenerology and pharmacology specialist for specialization of clinical pharmacology is one of the first generations for basic specialization in this field in Balkans. She finished post-graduation and specialization studies in the University of Novi Sad. Currently, she works in UCC in Prishtina from 1981; as a specialist of clinical pharmacology she has treated for 4 years patients with chemotherapy near Internal Clinic – Department of Hematology in UCC. She led for many years the laboratory for magisterial preparations in the Center Pharmacy and Dermatovenerology Clinic near CUU in Prishtina. She worked for some years in the Institute of Biochemistry near Diagnostic Center of CUU. Since 2001 she is working as a Clinical Dermatovenerologist and Pharmacologist in KDV and now leads Dermatological polyclinic and laboratory for magisterial preparations for several skin diseases and and aesthetic issues by which she treats patients in the polyclinic “GENTIANA GreLorGen”. She participated in many conferences and congresses in region and Europe with several scientific works in the field of clinical Dermatology, Oncology and Pharmacology and also as a lecturer of chemotherapy in the National Congress of Oncologists in Prishtina. She is a member of EADV and Kosovo Association of Dermatovenerologists and Oncologists.

Abstract:

The changes in the skin in the form of brown spots (hyperpigmentation) at humans, pose an aesthetic problem but also more a health problem/issue. Hyperpigmentation is presented in most of cases in the face region, but they do not spare other body also. During my research and the treatment of my patients, in my professional experience I have gone through patients with hyperpigmentation with different etiologies. Hyperpigmentation have multiethiology (conclusion). In the most of cases, they are signs that indicate and support us to find many internal disorders of the body and organism, just as an ID reaction to many diseases as: Endocrine (hormonal disorder), fungal infections, autoimmune disease (Morphea), therapeutic (either systemic or local), application of cosmetic preparations, and combined. In the time period July 2010 – 2015, in the clinic “GENTIANA GreLorGen” in Prishtina (Republic of Kosova), there have been treated in total 378 patients for various forms of hyperpigmentation, from whom, 34 male (9%) and 344 female (91%). These patients, in this research have been categorized in groups based in the etiology: Group I: Patients with Endocrine etiology (hormonal disorders); Group II: Patients with Autoimmune disease etiology; Group III: Patients with Mycotic (fungal) infections etiology; Group IV: Patients with Local and systemic therapeutics etiology; Group V: Patients with Cosmetic etiology; Group VI: Patients with different/combined etiology. Depending on the hyperpigmentation etiology, beside the local therapy with magisterial preparations, proper field specialists have been consulted as: Endocrinologist, Gynecologist, Cardiologist, and Rheumatologist. In addition, beside the local treatment with magisterial preparations as personal cream: ALBA-HYNOGEN, ALBAHYNOGEN M2, LORGEN SUN DAY, PREVITA-GEN, DEPEOR-KET, DEPEOR-GEN, ITRA-PEN, LOTTI FLU GEN, JORA-XH, PERIORB GEN, ANTISEP-CONT, ANTISEP-V; Patients have been treated with additional physical therapy as: MKDB (Micro dermo abrasion) combined with therapy. Conclusion: Hyperpigmentation treatment requires a proper multidisciplinary research and diagnostification, depending on its etiology and appearance

Biography:

Vesna Grivcheva-Panovska has received her Tenured Professorship in Dermatology in 2013 at University Saints Cyril and Methodius in Skopje, Macedonia. She is the Head of the Unit of Allergy and Clinical Immunology at the University Clinic of Dermatology and she is In-charge of Clinical Diagnostics. She has been working in the field of HAE since 1984, publishing numerous papers in international journals and took active participation in six international scientific projects.

Abstract:

Hereditary angioedema (HAE) is a rare genetic, life-threatening disease. Patients affected by HAE most of the time are without visible signs of the disease, besides the attacks, which can be brutal, painful and lethal. Several therapeutic approaches have been developed and used with various success, recombinant human C1INH (rhC1INH) being the most innovative current approach. Recombinant human C1INH is a purified derivative from rabbit milk which expresses the gene that encodes the synthesis of C1INH. The amino acid sequence of the recombinant form is identical to the human C1INH. C1INH is single-chain plasma glycoprotein with a molecular mass of 73,650 belonging to the super-family of serine protease inhibitors in plasma. C1INH is the only known inhibitor of activated plasma subcomponents C1s and C1r of the complementary component 1 of the complementary cascade classical pathway. Furthermore, C1INH inhibits the Manan-associated serine protease 2 (MASP2) of the lectin pathway of the complement. Additionally, it is the main inhibitor of the activated factor XII, factor XI and kallikrein of the plasma contact system. The rhC1INH was primarily developed for treatment of acute angioedema attacks in patients with HAE due to C1INH activity deficiency. The rhC1INH inhibitory potential of target proteases C1s, kallikrein, factor XIa and factor XIIa is highly comparable to the endogenous human C1 esterase inhibitory potential in vitro. Additional data of the efficacy are obtained by multiple analysis of primary efficacy sensitivity end point as well as the results of secondary and explorative efficacy.

Biography:

G Wiselin Jiji has completed his PhD from Anna University and Postdoctoral studies from University Medical Centre, Hamburg, Germany. She is the Research Director of Computer Science & Engineering in Dr. Sivanthi Aditanar College of Engineering, Tiruchendur. She has published more than 55 papers in reputed journals and has been serving as an Editorial Board Member of repute. She had completed 14 research projects in the area of Bio Medical imaging, Pattern Recognition and Remote Sensing Images. Her area of interest is neural network, image processing & medical imaging. She has received 12 National Awards and got HRD fellowship from Indian Council for Medical Research, New Delhi.

Abstract:

Identifying dermatological diseases is a challenging task nowadays. The Architecture employed three phases of operation to perform efficient skin lesion diagnosis. In the first phase, segmentation of Lesion region is calculated. Second Phase carries Color, Texture and shape feature vectors extraction. Iteratively calculated SVM weight values to all combination of feature sets and selected feature sets having highest significant p-map value. Third phase is employed by multi-class PSO Classification technique. Experiments on 20 classes of 1450 images are analyzed and results are evaluated using receiver operating characteristic (ROC) curve, which proved that the proposed architecture is highly contributed to computer-aided diagnosis of skin lesions. Our empirical evaluation has a superior retrieval and diagnosis performance when compared to other works.

Biography:

Sushruth G Kamoji graduated (MBBS) from Jawaharlal Nehru Medical College, Belgaum and went on to pursue his Post Graduate Diploma in Dermatology from the prestigious Madras Medical College, Chennai. After completing his Post Graduation, Dr Sushruth G. Kamoji went on to do his Fellowship in Dermato-Surgery from the renowned Post Graduate Institute of Medical Sciences (PGIMER), Chandigarh. Dr Sushruth now works as a Resident Dermatologist at a Govt. medical college – Belgaum Institute of Medical Sciences, Belgaum.

Abstract:

Background: Acne vulgaris is a chronic inflammatory disease affecting majority of the adolescent population. It has been shown to have a severe impact on the psychosocial well being of a person. Objectives: To assess the psychological impact of acne vulgaris, to analyze their beliefs and outlook about treatment of acne. Materials & Methods: A total of 390 cases of acne attending a government tertiary hospital and private skin clinic were analyzed. After obtaining their consent each of them were handed a self administered questionnaire and their grade of acne noted by the investigator. Patients were analyzed using Cardiff Acne Disability Index for their psychological impairment and another set of questions to determine their beliefs on cause, monthly budget for treatment and preference for topical and oral medications in treatment of acne. Results: Out of 390 cases 194 (49.7%) were males and 196 (50.3%) were females. Majority (364-93.33%) of them were students. Of these 390 cases, 321 (82.3%) had either Grade-1 or Grade-2 acne while there were only 9 cases of Grade-4 acne. With regard to the CADI scores 59 cases had a score of >8, signifying psychological impact on due to acne. However, the overwhelming majority (84.87%) had a CADI score <8. This is in contradiction to most studies conducted worldwide. A large number of respondents said that they had acne on and off but persistent acne for more than a year was noted in 121 (31%) cases. Despite this 206 (52.82%) cases had never sought treatment for acne. In the remainder of 184 patients (47.17%) who took treatment, 82 of them had used the medication based on an advertisement, friend’s suggestion or an over the counter product. It was quite naturally that 110 (59.78%) cases were not satisfied with the treatment. Even though 102 cases were treated by doctors only 50 had been explained about the usage of medicines and course of the disease which is very important to maintain adherence in acne therapy. When questioned about the cost of therapy, 136 (34%) respondents said they would not want to spend more than INR 300 per month for treatment of acne. 144 (37%) patients said they wished to use only a night cream for their treatment. 276 (71%) patients considered, dermatologist was the right person to treat acne followed by their family doctor (13.58%). Acne was considered to be a cosmetic problem by 149 (38.2%). 214 (54.87%) cases felt their acne was due to hormones. 169 (43.33%) cases attributed their flare in acne to stress.

Biography:

Umit Tursen has graduated from Gazi University as Medical Doctor. He has completed the Residency period of Dermatology in Ankara University, Faculty of Medicine and Department of Dermatology during 1995-1999. He has been working in Mersin University, School of Medicine, and Department of Dermatology since 2000. He is the Professor of Mersin University, School of Medicine, and Dermatology Department. He has published more than 80 papers in reputed journals, also books and serving as an Editorial Board Member of repute. He won Behcet’s Disease Research Award of “The scientific and technical research council of Turkey (TUBITAK)” in 1999.

Abstract:

Ingrown toenail is an often painful clinical condition that usually affects the big toe. Chemical matricectomy with phenol has a low recurrence rate and good cosmetic results. However, it produces extensive tissue destruction that can result in drainage and delayed healing. Alternatives such as sodium hydroxide and trichloroacetic acid (TCA) have therefore come into vogue. A total of 39 patients with 56 ingrown toenail edges underwent chemical matricectomy with 90% TCA after partial nail avulsion. In most of the patients, adverse effects such as postoperative pain and drainage were minimal. One patient who underwent matricectomy had recurrence in a single nail edge (1.8%) at 12 months follow-up. No recurrence was observed among 38 patients during the mean follow-up period. This was considered to be statistically significant (P<0.001). Partial nail avulsion followed by TCA matricectomy is a safe, simple and effective method with low rates of postoperative morbidity and high rates of success.

Biography:

Farrokh Khosravi has completed his Fellowship of Rheumatology in Shiraz University of Medical Sciences. He is a part time Assistant Professor of Medicine. His field of interest is autoimmune skin diseases and he has published papers in the field of Behcet's disease and Multicentric Reticulohistiocytosis in reputed journals and served as a Reviewer for Journal of Hormozgan University of Medical Sciences and Rheumatology Research Journal.

Abstract:

In this talk recent advances in understanding of drug induced fractures will be discussed. Fractures are the serious problem of the aged population, which is responsible for significant amount of morbidity and mortality. Any attempt to prevent or decrease incidence of fractures will save lives. Drugs increase Osteoporosis and Sarcopenia which both have a role in increasing fracture incidence. Sarcopenia is characterized by progressive and generalized loss of muscle mass and strength.

Biography:

Thomas de Aquino Paulo Filho completed his graduate course in Medicine from Rio Grande do Norte University in Brazil in 1982 and Post-graduate course at Sao Paulo University in 1985. He lived for ten years in Rio de Janeiro Brazil where he worked with public health with Leprosy and Sexual Transmitted Diseases. In 1994, he began to work at Department of Clinical Medicine and Dermatology at Onofre Lopes Hospital in Natal, Brazil and at Rio Grande do Norte University as Assistant Professor of Dermatology. He has published an Atlas of Cutaneous Cytology in 2007 for Brazilian Society of Dermatology and article on Derm101 website on Dermatology Practical and Conceptual Journal in October, 2014. He has worked with Cutaneous Cytopathology since 1986, collecting slides from tumoral and inflammatory-granulomatous cutaneous diseases. Recently, he has showed the importance of differential diagnosis between pigmented basal cell carcinoma and melanoma using Tzanck test.

Abstract:

Unfortunately, the cytopathology exam of the skin is not practiced by physicians in general dermatology. To take a fragment of the skin is very easy technique by cutaneous biopsy. In 1947, Dr. Tzanck in France had introduced the study of cutaneous cytology by publishing the view of pemphigus cells. Cutaneous cytology is an excellent, practical and cheap diagnosis method. We can take a correct diagnosis in few minutes. It is a complementary test comparing with histopathology test. Many cutaneous diseases can be diagnosed quickly by this exam with high accuracy. In 1960 in Brazil, Dr. Estevam Almeida Neto was a pioneer at Sao Paulo University, Department of Dermatology when he published a thesis on Leprosy Cytology during his doctorate course. While I was doing my Residence in Dermatology at Sao Paulo University, Dr. Estevam Almeida Neto had taught me Cutaneous Cytology and the process to take sample slides for examination. In my speech, I intend to show the importance of this exam and my experience for many years.

Biography:

Yuzhi Jiang has completed his PhD from The Second Military Medical University, China and Post-doctoral Studies from School of Medicine, Shanghai Jiao Tong University, China. He has published more than 15 papers in reputed journals.

Abstract:

The dermal tissues are composed of collage fibers and have been researched many years. The composite and three dimensional structure of collagen protein, collagen fibrils, collagen fiber has been clarified, however, the three dimensional structure of the extracellular matrix of dermal tissues has not been known, which impede the development of skin tissue engineering and skin regeneration. For example, the current skin tissue engineering products use the non-woven structure as the scaffold, which confirmed unsatisfactory after clinic usage. The other question is how the collagen fibers wove into the normal ECM during ontogeny and after wound healing is still unknown. This research will focus on the exact three dimensional mesoscopic structures of dermal tissues and would like to uncover the mechanism of the knit of collagen fibers, provide the golden standard to evaluate the skin tissue engineering product and regulate the wound healing.

Biography:

Avner Shemer has received his Medical Degree from Tel Aviv University, Israel. He has completed his Internship at Rambam Hospital, Haifa and received Training in Mycological Laboratory in Ramban Hospital and Guy’s Hospital, London. He has over 100 publications and recently published his new book “Superficial Mycoses” which offers an extensive and practical overview in the topic of superficial mycoses.

Abstract:

Introduction: Onychomycosis is a fungal infection of the nail plate and nail bed. Onychomycosis treatment options include oral and topical antifungal agents, devices and mechanical treatments such as nail avulsion or debridement. Methods: Participants were allocated into one of three treatment arms: Holes with combined oral and topical terbinafine, holes with topical terbinafine and topical terbinafine. An automatic device was used to drill two horizontal lines of holes just proximal to the border between the diseased and healthy portions of the nail with a diameter of 1.8-2 mm apart. Participants in Group-1 were instructed to take 250 mg of terbinafine per day for two weeks and participants in Groups-1, 2 and 3 were instructed apply terbinafine 1% spray. At each visit, nail specimens were collected and the percentage of infected nail that appeared healthy and clear was measured by a trained dermatologist. Results: A total of 98 people participated in this study (106 infected nails). Approximately 61% of the sample had 50% involvement and 38.7% had 75% involvement. There were no significant differences in baseline characteristics between treatment groups (Ps>0.05). The holes with topical terbinafine arm (Group-2) had the highest compliance rate, 95.6%, compared to 73.9% in the holes with oral and topical terbinafine (Group-1) and 73.7% in the topical terbinafine only arm (Group-3) and this difference was significant (P=0.013). The average percent clear nail in each treatment was well tolerated. Overall, nail drilling appears to be a safe and effective addition to onychomycosis treatment.

Biography:

Liyan Xi has completed her MD and PhD from Chinese Academy of Medical Sciences, Peking Union Medical College. She is the Vice-Chairperson of Chinese Society for Medical Mycology and the Vice-Chairperson of the Committee of Guangdong Medical Society for Dermatologist. She has published 51 scientific papers in reputed journals and has been serving as an Editorial Board Member of many journals focused on Medical Mycology and Dermatology.

Abstract:

Chromoblastomycosis as a chronic, cutaneous and subcutaneous infection has been reported more than 600 cases in China, but it has not been known to cause outbreaks until now. Several dematiaceous fungi are involved with the disease etiology, Cladophialophora carrionii is the most common agent in the North China, while Fonsecaea monophora instead of F. pedrosoi and F. nubica is the most common agent in the southern region. Infection commonly initiated after the etiologic agents gain through puncture wounds and mostly involved extremities of the males. Clinically, the lesions are polymorphic, including plaque, tumoral, cicatricial, verrucous, pseudo-vacuole, eczymatous and mixed type, which should be differentiated from some diseases associated with similar manifestation. The factors related to successful therapy for chromoblastomycosis might be pathogens, manifestations and severity. The common treatments include chemical therapy (systemic antifungal agents single used or combined) and physical methods, such as surgery, thermotherapy and cryotherapy. Patients with mild type of lesions could be treated successfully with systemic antifungal mono-therapy or combined therapy. But it is extremely difficult to eradicate severe lesions which are recalcitrant and disabling. Photodynamic Therapy (PDT) as a minimally invasive approach combines a non-toxic photoactivatable dye as photosensitizer with harmless visible light of correct wavelength to generate reactive oxygen or molecules that are toxic to target cells. It has recently been applied to the drug-resistant or recalcitrant chromoblastomycosis and showed considerable effects with promising future.

Biography:

Wajieha Saeed has completed her FCPS from College of Physicians & Surgeons Pakistan, Pakistan. She is a Senior Registrar at Dermatology Department, Mayo Hospital, Lahore and she is a Member of Pakistan Association of Dermatologist.

Abstract:

Acne has a prevalence of more than 90% in adolescents and persists into adulthood in almost 12-14% of cases with psychological and social stress of high intensity. There are many treatment options available for acne scars. Carbon dioxide (CO2) ablative fractional resurfacing is a new modality in laser techniques, for acne scars with more effectiveness and low side effect profile. An analytical experimental study was carried out at KEMU/Mayo Hospital, Lahore. Thirty patients of either sex with skin phototype IV & V, having moderate to severe atrophic scars were enrolled. Pre-treatment assessment of acne scars severity was done using Goodman & Boran qualitative scarring grading system. Patients were treated at 4 weeks internal with a maximum of 5 sessions. Follow up was done one month after the last session. Objective assessment was obtained at each session and graded according to percentage reduction in acne scars on a 4 point scale as excellent (>75% reduction), good (50-70% reduction), fair (25-50% reduction) and poor (<25% reduction). Side effects were noted at each session. Digital photographs were taken at the baseline, at each session and at follow up and were compared. The mean age of the patients was 21.6±2.2 years. There were 10 (33.33%) male and 20 (66.66%) female patients. A significant improvement in atrophic scars was observed. 12 (40%) patients showed good efficacy and 18 patients had fair efficacy. Erythema and edema followed by mild crusting was seen in all patients, which are cleared in 3-7 days with topical antibiotic. Very few patients developed post inflammatory hyper pigmentation.

Biography:

Yan Zheng is a Professor, Chief Physician, and Doctoral Tutor. She has completed her Bachelor's degree from the Department of Clinical Medicine, Xi'an Medical University and Doctor's degree from the Department of Dermatology, Second Affiliated Hospital of Xi'an Jiaotong University and did Postdoctoral Research for 2 years. She has published and received more than 70 papers, among which over 30 were SCI papers (Journal of Investigative Dermatology, Nature Communications, Experimental Dermatology, Immunology, BJD, Plos one, Dermatology, EJD, etc) and was quoted by the world authoritative magazine (Nature Review Immunology, the IF : 30). She is also the Reviewer of BJD, PloS One and IJD. As Deputy Editor or involved Editor, she has participated in the writing 7 books.http://dermatology.conferenceseries.com/abstract-submission.php

Abstract:

Cutaneous squamous cell carcinoma (cSCC) is one of the most common skin malignant tumors with an increasing incidence. Studies have shown that Yes-associated protein (YAP) participates in the development of a variety of tumors as an oncogene, but its role in cSCC has not been reported. In this study, we showed by immunohistochemistry that YAP expression was elevated in cSCC samples of different stages versus the normal skin and was well correlated with the progression of the disease. Down-regulation of YAP in cSCC cell lines A431 and SCL-1 inhibited cell proliferation by inducing a G1/S growth arrest, promoted apoptosis and reduced invasion and migration abilities in vitro. Conversely, overexpression of YAP promoted cell proliferation and protected cells against basal and chemodrug-induced apoptosis. These oncogenic effects of YAP were associated with activation of RAS protein and its downstream AKT and ERK signaling. Using a mouse xenograft model, we further demonstrated that YAP depletion inhibited cSCC tumor growth in vivo. Our results suggested that YAP is involved in the carcinogenesis and development of cSCC and may serve as a biomarker or therapeutic target of this disease.

Biography:

Abstract:

Background & Objectives: Leg ulcers are chronic wounds with varied causes and great medical impact. Management is complex and is associated with high costs for both patients and public health services, especially in low income-countries. In this study, we investigate the effect of autologous platelet rich fibrin (PRF) on healing of chronic non healing ulcers. Methods: A total of 37 patients with chronic non healing ulcers attending dermatology outpatient clinic were included in study population. A total of 43 ulcers were treated with PRF dressings. The patients were followed up on weekly basis for three months. Swab cultures were taken upon initial visit and then on weekly basis. The judgment criteria’s were the relative regression of the wound surface area and relative reduction of sloughy tissue. Results: Mean healing time was 35±4 days. 76.5% of ulcer became sterile during first week. Conclusion: PRF is simple and an effective treatment for chronic non healing ulcers leading to significant reduction in the time of healing. This study also strongly supports antibacterial effect of platelet rich products. Being autologous, PRF is safe and can be prepared without any sophisticated instruments with minimal cost and hence reducing the economic burden. Limitations: Diversity in the sites and causes of ulcers. Analysis to validate that the composition of PRF did not differ from case to case was not carried out.