Clinical & Experimental Dermatology

Biography

Biography: Krystyna Pienkowska

Abstract

Skin diseases and dermatology problems depend on many factors. One of the reasons is the presence of various substances in medical products on skin, personal care products or cosmetics which are not indifferent for skin. For example, active pharmaceutical ingredients API and excipients can irritate the skin and cause allergic reactions, e.g. itchy rash, eruptions, atopic skin disease, other. However, sometimes patients or consumers do not have to feel any reaction but API or excipients can irreversibly change skin barrier. This is a very negative effect. First of all, the skin does not sufficiently prevent the entrance of noxious compounds or microorganisms. The damage the skin structure can cause to increase of the uncontrolled penetration into the stratum corneum and even permeate into the deeper layers of the skin of substances which can be toxicity. To the other hand, destruction of the organization the human skin structure impacts on the loss of semi-permeable skin barrier function for activity substances which are applied on the skin in treatment different diseases. The increase penetration, permeation and percutaneous substances absorption can be disturb of kinetics of drug delivery through the skin at a optimal rate. Apart from these, some chemical substances (as excipients) can cause to cytotoxic effect on cells. A new interdisciplinary approach is presented to demonstrate possibility of penetration the low molecular linear and cyclic methyl siloxanes (silicones) to human stratum corneum and permeation to the deeper layers of the skin (epidermis and dermis). On the basis of the fluorescence microscope as well as ATR FT-IR spectroscopy both the penetration and permeation to this skin layer as well as impact on damage of corneocytes of the stratum corneum was observed. It will be shown that the phenomenon alteration the skin barrier was due to damage of stratum corneum structure in conjunction with distinct disturbances in the lipid structure the mortar lipid of the stratum corneum. The results present that cyclic and linear methyl siloxanes are able to overcome the barrier of the skin due to interaction between silicones and bilayers SC lipids. At the end of the oral presentation the results concerning the cytotoxic effects for immortal keratinocytes cell line HaCaT are presented. These conclusions are very important for the safety of using these compounds, particularly in dermatology and toxicology. Low molecular linear and cyclic methyl siloxanes are commonly used as excipients in medical products for skin (e.g. Rozex Metronidazolum) and personal care products (e.g. Penaten Baby or Body Lotion Garnier). Apart from this they are used across a wide spectrum of personal care applications, including antiperspirants, skin, hair and nail care formulations, grooming products, sun protection products, personal lubricants, and cosmetic preparations. It causes the significant human exposure to this group of compounds. They can be applied both by adults, children and infants. It must notice that so far, the transdermal route has not been described in the literature as a possible route of penetration and permeation by the linear and cyclic methyl siloxanes. ACKNOWLEDGMENTS This project was supported by Polish Ministry of Science (The National Centre for Research and Development NCN, grant 2013/11/B/NZ7/02076)