Boglarka Balazs
University of Szeged
Hungary
Title: Protein structure is changed in psoriatic skin on the unaffected region – imaging possibility with ATR-FTIR spectroscopy
Biography
Biography: Boglarka Balazs
Abstract
Psoriasis is a T lymphocyte-mediated inflammatory disorder that affects the skin. A number of studies have demonstrated the occurrence of lipid alterations in psoriatic skin, resulting in a highly perturbed stratum corneum (SC). Relatively little attention has been paid to the protein conformation of the SC. In this study, the Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectrum of the untreated psoriatic patients unharmed SC was obtained after tape stripping. We focused on the amide-I band components in order to establish whether there are any protein alterations in the intact areas of psoriatic skin. Fourier self-deconvolution (FSD) of the amide-I band was followed by curve-fitting to generate the underlying components. Integration of band areas provided an estimate of the secondary structure. The results indicated decreases in all amide-I band components, the peak at 1660 cm-1 revealing the most dramatic change. The intensity of the peak at 1660 cm-1 decreases almost to zero in psoriatic patients for both males (p < 0.05*) and females (p < 0.01**). This peak is characteristic of the turn structure in the protein chain. The decrease is marked in the case of the β-sheet structure at 1630 cm-1 too. The protein structure is changed on the unaffected area in the psoriatic skin disease. This ATR-FTIR imaging is a rapid and simple noninvasive method promotes a better understanding of the disease, and would be helpful in following the treatment.