Tae-Aug Kim
CHA University, South Korea
Title: Preventive effect of cacao extract on UVB-induced skin wrinkle formation via inhibition of DNA methylation
Biography
Biography: Tae-Aug Kim
Abstract
Cacao beans contain various bioactive phytochemicals that can attenuate or delay the onset of disease condition. However, the effect of cacao powder (CP) on UVB-induced wrinkle formation and the molecular mechanisms responsible has not previously been explored. Here, we report that oral administration of CP attenuates UVB-induced skin wrinkling by regulating genes involved in dermal matrix production and maintenance. Transcriptome analysis revealed that 853 genes are down- or up-regulated with CP supplementation, compared with UVB-irradiated mouse skin controls. CP elicited anti-wrinkle effects via inhibition of UVB-induced MMP-1 expression in a human skin equivalent model and human dermal fibroblasts (HDFs). Inhibition of UVB-induced AP-1 via CP supplementation is likely to affect the expression of MMP-1. In addition pattern-matched analysis of transcriptome and DMA methylome provide many new signature molecules regulated by CP and UVB. CP specifically modulated the expression of profilin-1 and involucrin by suppression of their DNA methylation. These results suggest that cacao extract may offer a protective effect against the photoaging process by epigenetic regulation of signature molecules, leading to an overall reduction in wrinkle formation.